SMAD4 antibody (AA 323-552)

Catalog No. ABIN1860591

This anti-SMAD4 antibody is a Rabbit Polyclonal antibody detecting SMAD4 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for SMAD4 antibody (AA 323-552) (ABIN1860591)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 323-552

Purpose: Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

Specificity: The antibody is a rabbit polyclonal antibody raised against Smad4. It has been selected for its ability to recognize Smad4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB925Hu01Recombinant Mothers Against Decapentaplegic Homolog 4 (Smad4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: Smad4

Background: DPC4, JIP, MADH4, Deletion target in pancreatic carcinoma 4

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy