LTA antibody (AA 49-194) (APC)

Catalog No. ABIN1860795

This anti-LTA antibody is a Guinea Pig Polyclonal antibody detecting LTA in WB, IHC, IP and ICC. Suitable for Rabbit.

Quick Overview for LTA antibody (AA 49-194) (APC) (ABIN1860795)

Target: LTA (Lymphotoxin-alpha (LTA))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This LTA antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LTA Antibody (APC)

Binding Specificity: AA 49-194

Purpose: Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GH STLKPAAHLV GDPSAQDSLR WRANTDRAFL RHGFSLSNNS LLVPSSGLYF VYSQVVFSGE GCSPKAVPTP LYLAHEVQLF SSQYSFHVPL LSAQKSVCPG PQGPWVRSVY QGAVFLLTQG DQLSTHTDGI AHLLLSPSSV FFGA

Specificity: The antibody is a cavia polyclonal antibody raised against TNFb. It has been selected for its ability to recognize TNFb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA134Rb01Recombinant Tumor Necrosis Factor Beta (TNFb)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: TNFb

Target Type: Chemical

Background: LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process