FLT4 antibody (AA 30-326)

Catalog No. ABIN1860935

The Rabbit Polyclonal anti-FLT4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect FLT4 in samples from Mouse.

Quick Overview for FLT4 antibody (AA 30-326) (ABIN1860935)

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-FLT4 Antibody

Binding Specificity: AA 30-326

Purpose: Polyclonal Antibody to Vascular Endothelial Growth Factor Receptor 3 (VEGFR3)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- P TLNITEDSYV IDTGDSLSIS CRGQHPLEWT WPGAQEVLTT GGKDSEDTRV VHDCEGTEAR PYCKVLLLAQ THANNTGSYH CYYKYIKARI EGTTAASTYV FVRDFKHPFI NKPDTLLVNR KDSMWVPCLV SIPGLNITLR SQSSALHPDG QEVLWDDRRG MRVPTQLLRD ALYLQCETTW GDQNFLSNLF VVHITGNELY DIQLYPKKSM ELLVGEKLVL NCTVWAEFDS GVTFDWDYPG KQAERAKWVP ERRSQQTHTE LSSILTIHNV SQNDLGPYVC EANNGIQRFR ESTEVI

Specificity: The antibody is a rabbit polyclonal antibody raised against VEGFR3. It has been selected for its ability to recognize VEGFR3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: VEGFR3 (AA 30-326)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FLT4

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGFR3

Background: FLT41, FLT4, PCL, Fms-Related Tyrosine Kinase 4, Tyrosine-protein kinase receptor FLT4

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling