Cytokeratin 18 antibody (AA 1-159)

Catalog No. ABIN1862828

The Rabbit Polyclonal anti-Cytokeratin 18 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Cytokeratin 18 in samples from Pig.

Quick Overview for Cytokeratin 18 antibody (AA 1-159) (ABIN1862828)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: AA 1-159

Purpose: Polyclonal Antibody to Cytokeratin 18 (CK18)

Specificity: The antibody is a rabbit polyclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB231Po01Recombinant Cytokeratin 18 (CK18)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: CK18

Background: KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

Pathways: Apoptosis, Caspase Cascade in Apoptosis