BHMT antibody (AA 11-314)

Catalog No. ABIN1866896

The Rabbit Polyclonal anti-BHMT antibody has been validated for WB, IHC, FACS, ICC, IP. It is suitable to detect BHMT in samples from Human.

Quick Overview for BHMT antibody (AA 11-314) (ABIN1866896)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-BHMT Antibody

Binding Specificity: AA 11-314

Purpose: Polyclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)

Specificity: The antibody is a rabbit polyclonal antibody raised against BHMT. It has been selected for its ability to recognize BHMT in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPG464Hu01Recombinant Betaine Homocysteine Methyltransferase (BHMT)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: Betaine-homocysteine S-methyltransferase

Pathways: Methionine Biosynthetic Process