ATP5J antibody (AA 1-108)

Catalog No. ABIN1867192

This Rabbit Polyclonal antibody specifically detects ATP5J in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for ATP5J antibody (AA 1-108) (ABIN1867192)

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ATP5J Antibody

Binding Specificity: AA 1-108

Purpose: Polyclonal Antibody to Coupling Factor 6 (CF6)

Specificity: The antibody is a rabbit polyclonal antibody raised against CF6. It has been selected for its ability to recognize CF6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: CF6 (Met1-Ser108)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: Coupling Factor 6

Background: ATP5J, ATP5A, ATP5, ATPM, ATP Synthase,H+ Transporting,Mitochondrial Fo Complex,Subunit F6, ATP synthase-coupling factor 6, mitochondrial

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process