ERN1 antibody (AA 571-832)

Catalog No. ABIN1867796

The Rabbit Polyclonal anti-ERN1 antibody has been validated for WB and IHC. It is suitable to detect ERN1 in samples from Human.

Quick Overview for ERN1 antibody (AA 571-832) (ABIN1867796)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ERN1 Antibody

Binding Specificity: AA 571-832

Purpose: Polyclonal Antibody to Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ERN1. It has been selected for its ability to recognize ERN1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1

Background: IRE1, IRE1P, Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase, Endoribonuclease

Pathways: ER-Nucleus Signaling, Unfolded Protein Response