HCCS antibody (AA 1-268)
Quick Overview for HCCS antibody (AA 1-268) (ABIN1868331)
Target
See all HCCS AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 1-268
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Purpose
- Polyclonal Antibody to Holocytochrome C Synthase (HCCS)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against HCCS. It has been selected for its ability to recognize HCCS in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Rat
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- Recombinant Holocytochrome C Synthase (HCCS) corresdonding to Met1~Ser268
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Isotype
- IgG
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Application Notes
- Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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Preservative
- ProClin
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 24 months
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- HCCS (Holocytochrome C Synthase (HCCS))
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Alternative Name
- HCCS
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Background
- CCHL, MCOPS7, Cytochrome C Heme-Lyase
Target
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