MECP2 antibody (AA 1-177)
Quick Overview for MECP2 antibody (AA 1-177) (ABIN1869144)
Target
See all MECP2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 1-177
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Purpose
- Polyclonal Antibody to Methyl CpG Binding Protein 2 (MECP2)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against MECP2. It has been selected for its ability to recognize MECP2 in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Mouse, Pig, Rat
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- RPC616Hu01Recombinant Methyl CpG Binding Protein 2 (MECP2)
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Isotype
- IgG
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Application Notes
- Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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Preservative
- ProClin
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 12 months
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- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
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Alternative Name
- MECP2
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Background
- RTS, AUTSX3, MRX16, MRX79, PPMX, RTT, Rett Syndrome, Mental Retardation,X-linked 16
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Pathways
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
Target
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