NFIX antibody (AA 13-298)

Catalog No. ABIN1869449

The Rabbit Polyclonal anti-NFIX antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect NFIX in samples from Rat.

Quick Overview for NFIX antibody (AA 13-298) (ABIN1869449)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-NFIX Antibody

Binding Specificity: AA 13-298

Purpose: Polyclonal Antibody to Nuclear Factor I/X (NFIX)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFIX. It has been selected for its ability to recognize NFIX in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor I/X (NFIX) corresdonding to His13~Thr298 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NFIX

Background: NF1A, CTF, CCAAT-Binding Transcription Factor, TGGCA-binding protein

Pathways: Skeletal Muscle Fiber Development