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Fibrillarin antibody

The Mouse Monoclonal anti-Fibrillarin antibody has been validated for WB, IP and IF. It is suitable to detect Fibrillarin in samples from Pichia pastoris.
Catalog No. ABIN187217

Quick Overview for Fibrillarin antibody (ABIN187217)

Target

See all Fibrillarin (FBL) Antibodies
Fibrillarin (FBL)

Reactivity

  • 55
  • 33
  • 32
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Pichia pastoris

Host

  • 52
  • 9
  • 1
Mouse

Clonality

  • 48
  • 14
Monoclonal

Conjugate

  • 41
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Fibrillarin antibody is un-conjugated

Application

  • 53
  • 19
  • 15
  • 13
  • 13
  • 8
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone

28F2
  • Specificity

    Species Reactivity: Yeast.

    Immunogen

    Mice were immunized with yeast nuclear protein preparations and hybridomas were screened against yeast cells (IF) and yeast protein homogenates (WB).

    Isotype

    IgG3
  • Application Notes

    Immunocytochemistry: Use at 1:1,000 - 1:10,000 Immunofluorescence: Use at 1:1,000 - 1:10,000 Immunoprecipitation: Assay dependent. Western blot: Use at 1:500 - 1:5,000 The optimal dilution for a specific application should be determined by the researcher.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    200 µl with 50% glycerol and sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C
  • Target

    Fibrillarin (FBL)

    Alternative Name

    FBL

    Background

    Nop1p is the yeast homologue of fibrillarin found in a wide variety of organisms. In yeast, Nop1p is localized to the nucleolus and is essential for cell viability. Nop1p is found in small nuclear ribonucleoprotein particles (snRNP) of the nucleolus and is required for many post-transcriptional steps in yeast ribosome biosynthesis.

    Pathways

    Ribonucleoside Biosynthetic Process
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