FAS antibody (FITC)

Catalog No. ABIN187286

Product Details anti-FAS Antibody (FITC)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Species Reactivity: Human. Others not tested.

Clone: 3-22

Isotype: IgG1

Application Details

Application Notes: Monitoring of activated T cells in peripheral blood, Analysis of NK subsets, Study of B cell activation. Flow cytometry Immunofluorescence PBMC: Add 10 µL of mAb/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 4 °C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Whole Blood: Add 10 µL of mAb/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (RT). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for lysed whole blood and immunofluorescence analysis with a flow cytometer or microscope. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1.0 mL of FITC-labeled monoclonal antibody in PBS, 0.08% sodium azide and protein stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Background: Activation of either the 55 kDa tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity