TLR9 antibody

Catalog No. ABIN187334

Product Details anti-TLR9 Antibody

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: Reacts with mouse TLR9. Weak cross-reactivity with human TLR9. Species Reactivity: Mouse and human. Others not tested.

Sterility: 0.2 μm filtered

Clone: 5G5

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Use at approximately 1:50 dilution. Immunoassays: (detection antibody) Use at approximately 1:50 dilution. Immunohistochemistry: (frozen / paraffin sections) stains RAW macrophages and TLR9 transfected HEK293 cells. Use at aproximately 1:50 dilution. Western blot: Use at approximately 1:50 dilution. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL of 100 µg/mL 0.2 µm filtered monoclonal antibody solution in PBS containing protein stabilizer and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: CD289 / TLR9 (TLR9 Products)

Background: Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense of many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. As investigated so far, all functional characterized TLR signals via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades