ABCC5 antibody

Catalog No. ABIN187353

This anti-ABCC5 antibody is a Rat Monoclonal antibody detecting ABCC5 in WB, IF and IHC (fro). Suitable for Human.

Quick Overview for ABCC5 antibody (ABIN187353)

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ABCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M5I-1

Product Details anti-ABCC5 Antibody

Specificity: M5I-1 reacts with an internal epitope of MRP5 (ABCC5), a 190-200 kDa transmembrane protein that is closely related to the multi-drug resistance protein MRP. M5I-1 does not cross-react with the human MDR1, MRP1, MRP2, or MRP3 gene products. Species Reactivity: Human. Others not tested.

Sterility: 0.22 μm filtered

Immunogen: A bacterial fusion protein containing amino acids 82-168 of human MRP5.Lymph node cells from an immunized rat (Wistar) fused with mouse myeloma (SP2/0) cells.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Optimal conditions still to be determined. Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-rat IgG and a monoclonal rat APAAP complex. Alternatively, after incubation with primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution (with anti-rat-HRP). The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: IMDM, supplemented with Nutridoma-NS/SP. Th

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for ABCC5

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Alternative Name: ABCC5 / MRP5

Pathways: Glycosaminoglycan Metabolic Process