PARP4 antibody (AA 491-494)

Catalog No. ABIN187362

The Mouse Monoclonal anti-PARP4 antibody (Clone P193-4) (ABIN187362) specifically detects PARP4 in WB, IHC (fro) and IF. The antibody is reactive with Human samples.

Quick Overview for PARP4 antibody (AA 491-494) (ABIN187362)

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: P193-4

Product Details anti-PARP4 Antibody

Binding Specificity: AA 491-494

Specificity: This clone (P193-4) reacts with an internal epitope (amino acids 491-494, HPGE) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.

Immunogen: Clone p193-4 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Isotype: IgG2b

Application Details

Application Notes: Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL or 0.5 mL of antibody solution containing approximately 250ug of immunoglobulin per mL. The solution contains 1% BSA and 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for PARP4

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Alternative Name: PARP4

Background: VPARP is an antibody to the minor vault protein.