BAFF antibody

Catalog No. ABIN187404

The Rat Monoclonal anti-BAFF antibody (Clone Buffy-1) (ABIN187404) specifically detects BAFF in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for BAFF antibody (ABIN187404)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: Buffy-1

Product Details anti-BAFF Antibody

Specificity: Recognizes the stalk region present in the membrane-bound form of human BAFF. Species Reactivity: Human, others not tested.

Immunogen: Human sol. BAFF (rec., aa. 83-285)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: optimal concentration range is 0.5-5 µg/ml. Western blotting The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/ml. Purity > 95% as determined by SDS-PAGE. The antibody was purified from concentrated hybridoma cell culture supernatant.

Buffer: 100 µg in PBS and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: CD257 / BAFF

Background: BAFF (for B-cell activating factor belonging to the TNF family), a new member of the tumor necrosis factor (TNF) family was simultaneously identified by four different laboratories and named BAFF, TALL-1, THANK, zTNF4. It is abundantly expressed in monocytes and macrophages, upregulated by interferon gamma. Endogenous BAFF is processed intracellularly by a protease of the furin family of proprotein convertases. Secreted BAFF acts as a potent B cell growth factor. Recently, two receptors for BAFF have been identified and termed as BCMA and TACI.

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response