APAF1 antibody

Catalog No. ABIN187407

The Rat Monoclonal anti-APAF1 antibody (Clone 2E12) (ABIN187407) specifically detects APAF1 in WB, IP, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for APAF1 antibody (ABIN187407)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2E12

Product Details anti-APAF1 Antibody

Specificity: Recognizes the CARD domain of human Apaf-1. Species Reactivity: Human, others not tested. Does not cross-react with mouse or rat Apaf-1.

Immunogen: FLAG-tagged recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry Immunohistochemistry: Electron microscopy on frozen sections. Immunoprecipitation Western blot ELISA The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL.

Buffer: 100 µg of liquid Protein G purified monoclonal antibody in PBS containing 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background: Programmed cell death (apoptosis) requires the activation of caspases, which are in turn regulated by adaptor proteins such as Apaf-1 (activator of apoptosis protease-activating factor-1). The 136 kDa protein Apaf-1 is a mammalian homolog of C. elegans/CED-4, and is required for some pathways of cell death. Apaf-1 promotes the aggregation and self-processing of the initiator caspase, caspase-9 in the presence of cytochrome c and dATP. It consists of four function-determining regions: (1) an N-terminal caspase activation and recruitment domain (CARD), which is required for binding to pro-caspase-9, (2) a central CED-4 homologous region, (3) an Apaf-1 specific region, and (4) 12 identified WD40 repeats at the C-terminus of the protein.

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity