HSP70 antibody

Catalog No. ABIN187606

This Mouse Monoclonal antibody specifically detects HSP70 in WB, IP, FACS and IHC (p). It exhibits reactivity toward Human, Cow and Monkey.

Quick Overview for HSP70 antibody (ABIN187606)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: W27

Product Details anti-HSP70 Antibody

Specificity: This clone recognizes both the constitutive (Hsp73) and inducible (Hsp72) forms of Hsp70. Species Reactivity: Reacts with Human, Monkey and Bovine. Other species not tested. Positive Control: BT474 or HeLa cells. Breast carcinoma.

Immunogen: Hsp70 protein from HeLa cells.Produced by fusion between BALB/c spleno-cytes and mouse myeloma NS-1 cells.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry Immunofluorescence (Not assessed) Immunoprecipitation: Use at 2 µg/mg protein lysate. Use Protein A. Western blotting: 0.5-1 µg/ml for 2 hrs. at RT Immunohistology: Formalin/paraffin - Use at 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Clone W27 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100ug/500ul in 10 mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Purified from ascites fluid by Protein A chromatography.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Heat shock protein 70 / HSP70

Background: A wide variety of environmental and patho-physiological stress conditions trigger the synthesis of a family of proteins known as heat shock proteins, more appropriately called stress response proteins. Many heat shock proteins, including members of the Ssp70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation and secretion. Hsp70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other unknown proteins. Also, Hsp70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs and other damaging conditions.