S1PR4 antibody (C-Term)

Catalog No. ABIN187788

The Rabbit Polyclonal anti-S1PR4 antibody (ABIN187788) specifically detects S1PR4 in WB. The antibody is reactive with Human samples.

Quick Overview for S1PR4 antibody (C-Term) (ABIN187788)

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-S1PR4 Antibody

Binding Specificity: C-Term

Specificity: Reacts with EDG-6. Species Reactivity: Human, other species not tested. Positive Control: RH7777 cells transfected with EDG-6 protein. The optimal dilution for a specific application should be determined by the researcher.

Sterility: 0.2 μm filtered

Immunogen: Synthetic peptide derived from the C-terminal of the EDG-6 receptor.

Application Details

Application Notes: Western Blot: Use at 5-10 µg/ml using a human EDG-6 receptor transfected cell line. Detects a band of approximately 45 kDa in Cos cells transfected with full length human EDG-6. Due to low expression of EDG receptors, we recommend using Pierce Femto Signal substrate for western blot development.

Restrictions: For Research Use only

Handling

Buffer: 100 µg purified antibody at 1 mg/ml in PBS with 0.08% sodium azide. Solution was sterile filtered at 0.2 µm.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for S1PR4

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Alternative Name: EDG-6 / S1P4

Background: EDG-6 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-6 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-6 is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG-6 receptor is the sphingosine 1-phosphate receptor-4 (S1P-4).