Cyclin B1 antibody (pSer35)

Catalog No. ABIN1881155

The Rabbit Polyclonal anti-Cyclin B1 antibody has been validated for WB and DB. It is suitable to detect Cyclin B1 in samples from Human. There are 3+ publications available.

Quick Overview for Cyclin B1 antibody (pSer35) (ABIN1881155)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Clone: RB42141

Product Details anti-Cyclin B1 Antibody

Binding Specificity: pSer35

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Cyclin B1 antibody (ABIN1881155)

Kreis, Sanhaji, Krämer, Sommer, Rödel, Strebhardt, Yuan: "Restoration of the tumor suppressor p53 by downregulating cyclin B1 in human papillomavirus 16/18-infected cancer cells." in: Oncogene, Vol. 29, Issue 41, pp. 5591-603, (2010) (PubMed).

van Zon, Ogink, ter Riet, Medema, te Riele, Wolthuis: "The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 587-602, (2010) (PubMed).

Harley, Allan, Sanderson, Clarke: "Phosphorylation of Mcl-1 by CDK1-cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest." in: The EMBO journal, Vol. 29, Issue 14, pp. 2407-20, (2010) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

Molecular Weight: 48337

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase