EIF3L antibody (N-Term)

Catalog No. ABIN1881291

Cited in 3+ publications. The Rabbit Polyclonal anti-EIF3L antibody (Clone RB41025) (ABIN1881291) specifically detects EIF3L in WB. The antibody is reactive with Human samples.

Quick Overview for EIF3L antibody (N-Term) (ABIN1881291)

Target: EIF3L (Eukaryotic Translation Initiation Factor 3, Subunit L (EIF3L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3L antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB41025

Product Details anti-EIF3L Antibody

Binding Specificity: AA 12-40, N-Term

Predicted Reactivity: B, C, M, X

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This EIF3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human EIF3L.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-EIF3L antibody (ABIN1881291)

Zhou, Sandercock, Fraser, Ridlova, Stephens, Schenauer, Yokoi-Fong, Barsky, Leary, Hershey, Doudna, Robinson: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, (2008) (PubMed).

Masutani, Sonenberg, Yokoyama, Imataka: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, (2007) (PubMed).

Damoc, Fraser, Zhou, Videler, Mayeur, Hershey, Doudna, Robinson, Leary: "Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 7, pp. 1135-46, (2007) (PubMed).

Target Details for EIF3L

Target: EIF3L (Eukaryotic Translation Initiation Factor 3, Subunit L (EIF3L))

Alternative Name: EIF3L

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 66727

NCBI Accession: NP_001229852, NP_057175

UniProt: Q9Y262

Pathways: Ribonucleoprotein Complex Subunit Organization