ISCU antibody (C-Term)

Catalog No. ABIN1881467

There are 5+ publications for this product available. The Rabbit Polyclonal anti-ISCU antibody is suitable to detect ISCU in samples from Human and Mouse. It has been validated for WB.

Quick Overview for ISCU antibody (C-Term) (ABIN1881467)

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISCU antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB35591

Product Details anti-ISCU Antibody

Binding Specificity: AA 138-167, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ISCU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the C-terminal region of human ISCU.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ISCU antibody (ABIN1881467)

Okerlund, Kivimäe, Tong, Peng, Ullian, Cheyette: "Dact1 is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 12, pp. 4362-8, (2010) (PubMed).

Chan, Zhang, Hemann, Mahoney, Zweier, Loscalzo: "MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2." in: Cell metabolism, Vol. 10, Issue 4, pp. 273-84, (2009) (PubMed).

Kollberg, Tulinius, Melberg, Darin, Andersen, Holmgren, Oldfors, Holme: "Clinical manifestation and a new ISCU mutation in iron-sulphur cluster deficiency myopathy." in: Brain : a journal of neurology, Vol. 132, Issue Pt 8, pp. 2170-9, (2009) (PubMed).

Huang, Dizin, Cowan: "Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe-S cluster scaffold protein." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 13, Issue 5, pp. 825-36, (2008) (PubMed).

Mochel, Knight, Tong, Hernandez, Ayyad, Taivassalo, Andersen, Singleton, Rouault, Fischbeck, Haller: "Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance." in: American journal of human genetics, Vol. 82, Issue 3, pp. 652-60, (2008) (PubMed).

Target Details for ISCU

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Alternative Name: ISCU

Background: Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Molecular Weight: 17999

NCBI Accession: NP_055116, NP_998760

UniProt: Q9H1K1