NXT1 antibody (C-Term)

Catalog No. ABIN1881591

Cited in 5+ publications. The Rabbit Polyclonal anti-NXT1 antibody (Clone RB30598) (ABIN1881591) specifically detects NXT1 in WB. The antibody is reactive with Mouse samples.

Quick Overview for NXT1 antibody (C-Term) (ABIN1881591)

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXT1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB30598

Product Details anti-NXT1 Antibody

Binding Specificity: AA 85-111, C-Term

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NXT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-111 amino acids from the C-terminal region of human NXT1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-NXT1 antibody (ABIN1881591)

Venkatesan, Rual, Vazquez, Stelzl, Lemmens, Hirozane-Kishikawa, Hao, Zenkner, Xin, Goh, Yildirim, Simonis, Heinzmann, Gebreab, Sahalie, Cevik, Simon, de Smet, Dann, Smolyar, Vinayagam, Yu et al.: "An empirical framework for binary interactome mapping...." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, (2008) (PubMed).

Forler, Köcher, Rode, Gentzel, Izaurralde, Wilm: "An efficient protein complex purification method for functional proteomics in higher eukaryotes." in: Nature biotechnology, Vol. 21, Issue 1, pp. 89-92, (2003) (PubMed).

Katahira, Straesser, Saiwaki, Yoneda, Hurt: "Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9242-6, (2002) (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Wiegand, Coburn, Zeng, Kang, Bogerd, Cullen: "Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes." in: Molecular and cellular biology, Vol. 22, Issue 1, pp. 245-56, (2001) (PubMed).

Target Details for NXT1

Target: NXT1 (NTF2-Related Export Protein 1 (NXT1))

Alternative Name: NXT1

Background: The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene.

NCBI Accession: NP_037380

UniProt: Q9UKK6