GRK2 antibody (C-Term)

Catalog No. ABIN1882061

Product Details anti-GRK2 Antibody

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Binding Specificity: AA 633-660, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1.

Clone: RB0982

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-GRK2 antibody (ABIN1882061)

Rao, Rapoport, Kim: "Decreased GRK3 but not GRK2 expression in frontal cortex from bipolar disorder patients." in: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), Vol. 12, Issue 6, pp. 851-60, (2009) (PubMed).

Eichmann, Lorenz, Hoffmann, Brockmann, Krasel, Lohse, Quitterer: "The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gbeta gamma binding site." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8052-7, (2003) (PubMed).

Li, Xiang, Su, Zhang, Huang, Ma: "Agonist-induced formation of opioid receptor-G protein-coupled receptor kinase (GRK)-G beta gamma complex on membrane is required for GRK2 function in vivo." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 30219-26, (2003) (PubMed).

Wan, Sambi, Tate, Waters, Pyne et al.: "The inhibitory gamma subunit of the type 6 retinal cGMP phosphodiesterase functions to link c-Src and G-protein-coupled receptor kinase 2 in a signaling unit that regulates p42/p44 mitogen-activated ..." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18658-63, (2003) (PubMed).

Yang, Zhang, Lai, Xing, Liu: "Pleckstrin homology domain of G protein-coupled receptor kinase-2 binds to PKC and affects the activity of PKC kinase." in: World journal of gastroenterology : WJG, Vol. 9, Issue 4, pp. 800-3, (2003) (PubMed).

Hagen, Kondyra, Grocott, El-Moalem, Bainbridge, Mathew, Newman, Reves, Schwinn, Kwatra: "Cardiopulmonary bypass decreases G protein-coupled receptor kinase activity and expression in human peripheral blood mononuclear cells." in: Anesthesiology, Vol. 98, Issue 2, pp. 343-8, (2003) (PubMed).

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: ADRBK1 (ADRBK1 Products)

Background: Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Molecular Weight: 79574

NCBI Accession: NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling