RENT1/UPF1 antibody (N-Term)

Catalog No. ABIN1882125

Product Details anti-RENT1/UPF1 Antibody

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Binding Specificity: AA 7-37, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RENT1/UPF1 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human RENT1.

Clone: RB8018

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-RENT1/UPF1 antibody (ABIN1882125)

Kang, Shen, Macdonald: "Two molecular pathways (NMD and ERAD) contribute to a genetic epilepsy associated with the GABA(A) receptor GABRA1 PTC mutation, 975delC, S326fs328X." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 9, pp. 2833-44, (2009) (PubMed).

Ohnishi, Yamashita, Kashima, Schell, Anders, Grimson, Hachiya, Hentze, Anderson, Ohno: "Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7." in: Molecular cell, Vol. 12, Issue 5, pp. 1187-200, (2003) (PubMed).

Mendell, ap Rhys, Dietz: "Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts." in: Science (New York, N.Y.), Vol. 298, Issue 5592, pp. 419-22, (2002) (PubMed).

Lykke-Andersen: "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8114-21, (2002) (PubMed).

Carastro, Tan, Selg, Jack, So, Downey: "Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta." in: Nucleic acids research, Vol. 30, Issue 10, pp. 2232-43, (2002) (PubMed).

Target Details for RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Alternative Name: RENT1 (UPF1 Products)

Background: RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.

Molecular Weight: 124345

NCBI Accession: NP_002902

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome