UCHL3 antibody (C-Term)

Catalog No. ABIN1882147

This Rabbit Polyclonal antibody specifically detects UCHL3 in WB and IHC (p). It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for UCHL3 antibody (C-Term) (ABIN1882147)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4187

Product Details anti-UCHL3 Antibody

Binding Specificity: AA 195-225, C-Term

Predicted Reactivity: B, M, Pig, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-UCHL3 antibody (ABIN1882147)

Henderson, Vij, Zeitlin: "Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11314-25, (2010) (PubMed).

Sun, Ying, Li, Shang, He, Chen, Cheng, Zhou: "Role of UCH-L1/ubiquitin in acute testicular ischemia-reperfusion injury." in: Biochemical and biophysical research communications, Vol. 366, Issue 2, pp. 539-44, (2008) (PubMed).

Saito, Iida, Sekine, Kawauchi, Higuchi, Ogawa, Nakamura: "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population." in: Journal of human genetics, Vol. 48, Issue 5, pp. 249-70, (2003) (PubMed).

Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss, Pohl: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, (1989) (PubMed).

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

Molecular Weight: 26183

NCBI Accession: NP_001257881, NP_005993

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation