ERK2 antibody (C-Term)

Catalog No. ABIN1882175

The Rabbit Polyclonal anti-ERK2 antibody has been validated for WB, IHC (p) and FACS. It is suitable to detect ERK2 in samples from Human. There are 5+ publications available.

Quick Overview for ERK2 antibody (C-Term) (ABIN1882175)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB11608

Product Details anti-ERK2 Antibody

Binding Specificity: AA 316-345, C-Term

Predicted Reactivity: B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MAPK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ERK2 antibody (ABIN1882175)

Munshi, Wu, Mukhopadhyay, Ottaviano, Sassano, Koblinski, Platanias, Stack et al.: "Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ..." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39042-50, (2004) (PubMed).

Mukherjee, Huang, Petkova, Albanese, Pestell, Braunstein, Christ, Wittner, Lisanti, Berman, Weiss, Tanowitz: "Trypanosoma cruzi infection activates extracellular signal-regulated kinase in cultured endothelial and smooth muscle cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5274-82, (2004) (PubMed).

Lou, Xie, Zhang, Yao, Luo, Wang, Shi, Yao: "Nek2A specifies the centrosomal localization of Erk2." in: Biochemical and biophysical research communications, Vol. 321, Issue 2, pp. 495-501, (2004) (PubMed).

Mitsushima, Suwa, Amachi, Ueda, Kioka: "Extracellular signal-regulated kinase activated by epidermal growth factor and cell adhesion interacts with and phosphorylates vinexin." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34570-7, (2004) (PubMed).

Huang, Chang, Liu: "Basic fibroblast growth factor antagonizes activin A-mediated growth inhibition and hemoglobin synthesis in K562 cells by activating ERK1/2 and deactivating p38 MAP kinase." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1247-52, (2004) (PubMed).

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1

Background: MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

Molecular Weight: 41390

NCBI Accession: NP_002736, NP_620407

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins