Sonic Hedgehog antibody (AA 151-162)

Catalog No. ABIN1882186

This Rabbit Polyclonal antibody specifically detects Sonic Hedgehog in WB. It exhibits reactivity toward Zebrafish (Danio rerio) and has been mentioned in 4+ publications.

Quick Overview for Sonic Hedgehog antibody (AA 151-162) (ABIN1882186)

Target: Sonic Hedgehog (SHH)

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB46723

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 151-162

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This shha antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 151-162 amino acids from the Central region of DANRE shha.

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Sonic Hedgehog antibody (ABIN1882186)

Müller, Chang, Albert, Fischer, Tora, Strähle: "Intronic enhancers control expression of zebrafish sonic hedgehog in floor plate and notochord." in: Development (Cambridge, England), Vol. 126, Issue 10, pp. 2103-16, (1999) (PubMed).

Zardoya, Abouheif, Meyer: "Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 13036-41, (1996) (PubMed).

Ekker, Ungar, Greenstein, von Kessler, Porter, Moon, Beachy: "Patterning activities of vertebrate hedgehog proteins in the developing eye and brain." in: Current biology : CB, Vol. 5, Issue 8, pp. 944-55, (1995) (PubMed).

Roelink, Augsburger, Heemskerk, Korzh, Norlin, Ruiz i Altaba, Tanabe, Placzek, Edlund, Jessell: "Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord." in: Cell, Vol. 76, Issue 4, pp. 761-75, (1994) (PubMed).

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: shha

Background: Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning, dorso-ventral patterning of the brain and early patterning of the developing eyes. Displays floor plate-inducing activity. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO (By similarity).

Molecular Weight: 46403

UniProt: Q92008

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development