NFKBIA antibody

Catalog No. ABIN1882268

The Mouse Monoclonal anti-NFKBIA antibody has been validated for WB. It is suitable to detect NFKBIA in samples from Human. There are 4+ publications available.

Quick Overview for NFKBIA antibody (ABIN1882268)

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFKBIA antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1121CT8-6-1

Product Details anti-NFKBIA Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged NFKBIA protein was used to produced this monoclonal antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-NFKBIA antibody (ABIN1882268)

Cockman, Lancaster, Stolze, Hewitson, McDonough, Coleman, Coles, Yu, Hay, Ley, Pugh, Oldham, Masson, Schofield, Ratcliffe: "Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 40, pp. 14767-72, (2006) (PubMed).

Jungnickel, Staratschek-Jox, Bräuninger, Spieker, Wolf, Diehl, Hansmann, Rajewsky, Küppers: "Clonal deleterious mutations in the IkappaBalpha gene in the malignant cells in Hodgkin's lymphoma." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 395-402, (2000) (PubMed).

Huxford, Huang, Malek, Ghosh: "The crystal structure of the IkappaBalpha/NF-kappaB complex reveals mechanisms of NF-kappaB inactivation." in: Cell, Vol. 95, Issue 6, pp. 759-70, (1999) (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, (1991) (PubMed).

Target Details for NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Alternative Name: NFKBIA

Background: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Molecular Weight: 35609

NCBI Accession: NP_065390

UniProt: P25963

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling