PKLR antibody (AA 1-230)

Catalog No. ABIN1885816

This Rabbit Polyclonal antibody specifically detects PKLR in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for PKLR antibody (AA 1-230) (ABIN1885816)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PKLR Antibody

Binding Specificity: AA 1-230

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 230 of Pyruvate Kinase(liver/RBC)

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.50-1.500
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Background: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis.Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]

Molecular Weight: 62 kDa

Gene ID: 5313

NCBI Accession: NM_000298, NP_000289

Pathways: Ribonucleoside Biosynthetic Process