OGT antibody (AA 213-462)

Catalog No. ABIN1886200

This Rabbit Polyclonal antibody specifically detects OGT in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for OGT antibody (AA 213-462) (ABIN1886200)

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-OGT Antibody

Binding Specificity: AA 213-462

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 213 and 462 of O-GlcNAc transferase

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: O-GlcNAc Transferase

Background: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues.Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrateof nearby sites by steric or electrostatic effects.The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal.Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq]

Molecular Weight: 116 kDa

Gene ID: 8473

NCBI Accession: NP_858059, NM_181673

Pathways: Regulation of Carbohydrate Metabolic Process