RHOA antibody (AA 101-150)

Catalog No. ABIN1886746

This Rabbit Polyclonal antibody specifically detects RHOA in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RHOA antibody (AA 101-150) (ABIN1886746)

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RHOA Antibody

Binding Specificity: AA 101-150

Immunogen: KLH conjugated synthetic peptide derived from human RhoA (101-150aa)

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.100-1.500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: -20 °C

Storage Comment: Store at -20 °C/1 year

Expiry Date: 12 months

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA

Background: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades.Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility.The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane.It is thought to be important in cell locomotion.Overexpression of this gene is associated with tumor cell proliferation and metastasis.Multiple alternatively spliced variants, encoding the same protein, have been identified.References: Graham D.L.et al.Chem.Biol.9:375-381(2002) Snyder J.T.et al.Nat.Struct.Biol.9:468-475(2002) Longenecker K.et al.Acta Crystallogr.D 59:876-880(2003)
Synonyms: RhoA protein, Aplysia ras related homolog 12, ARH 12, ARH12

Molecular Weight: 21 kDa

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome