Ki-67 antibody (N-Term)

Catalog No. ABIN1886792

The Rabbit Polyclonal anti-Ki-67 antibody has been validated for IHC, IF and ICC. It is suitable to detect Ki-67 in samples from Human.

Quick Overview for Ki-67 antibody (N-Term) (ABIN1886792)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Ki-67 Antibody

Binding Specificity: N-Term

Immunogen: KLH conjugated synthetic peptide derived from human Ki67 N-terminus

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.100-1.500
Immunohistochemistry 1.100-1.500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: -20 °C

Storage Comment: Store at -20 °C/1 year

Expiry Date: 12 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67

Background: Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase).It is absent in resting (G0) cells.Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index).In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index.The correlation between low Ki67 index and histologically low grade tumours is strong.Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.Sueishi M.et al.J.Biol.Chem.275:28888-28892(2000) Takagi M.et al.J.Biol.Chem.276:25386-25391(2001) Beausoleil S.A.et al.Proc.Natl.Acad.Sci.U.S.A.101:12130-12135(2004) Olsen J.V.et al.Cell 127:635-648(2006)
Synonyms: KIA, Ki-67, Ki67, MKI67

Molecular Weight: 358 kDa

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process