Glutathione Reductase antibody

Catalog No. ABIN7540298

This Rabbit Polyclonal antibody specifically detects Glutathione Reductase in WB, IP and IL. It exhibits reactivity toward Zea mays, Potato, Pisum sativum, Nicotiana tabacum, Barley and Arabidopsis thaliana and has been mentioned in 2+ publications.

Quick Overview for Glutathione Reductase antibody (ABIN7540298)

Target: Glutathione Reductase (GSR)

Reactivity: Zea mays, Potato, Pisum sativum, Nicotiana tabacum, Barley, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunolocalization (IL)

Product Details anti-Glutathione Reductase Antibody

Characteristics: Expected / apparent Molecular Weight of the Antigene: 54 kDa

Purification: affinity purified

Immunogen: maltose binding protein (MBP) fusion of Zea mays GR, O64409

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 5000 with standard ECL (WB), needs to be optimised.

Comment:

This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Concentration: 7 µg/ µL

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Glutathione Reductase antibody (ABIN7540298)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Sobrino-Plata, Ortega-Villasante, Flores-Cuaceres, Escobar, Del Campo, Hernuandez: "Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa." in: Chemosphere, Vol. 77, Issue 7, pp. 946-54, (2009) (PubMed).

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: Glutathione reductase (GR)

Background: AGI Code: At3g54660
Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).

Molecular Weight: 54 kDa

UniProt: O64409

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis