Rubisco antibody

Catalog No. ABIN190723

This Rabbit Polyclonal antibody specifically detects Rubisco in WB and IL. It exhibits reactivity toward Arabidopsis thaliana, Aucuba japonica, Barley, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Zea mays and Anabaena variabilis. It has been mentioned in 2+ publications

Quick Overview for Rubisco antibody (ABIN190723)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivity: Arabidopsis thaliana, Aucuba japonica, Barley, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Zea mays, Anabaena variabilis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubisco antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-Rubisco Antibody

Purpose: Anti-Rubisco | 557 kDa hexadecamer

Cross-Reactivity (Details): Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Not reactive in: Chlamydomonas reinhardtii (immulolocalization)

Predicted Reactivity: higher plants,algae,Anthoceros agrestis,Nannochloropsis sp.

Purification: Serum

Immunogen: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Application Details

Application Notes: 1 : 10 000-1 : 20 000 on 0,5-10 μg total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)

Comment:

RbcS subunit is not detected by this antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Rubisco antibody (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Target Details for Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Alternative Name: Rubisco

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Molecular Weight: 53-55 | 53-55 kDa

Gene ID: 2715621

UniProt: P00875, Q43832