Rubisco antibody
(2 references)-
- Target See all Rubisco (RBCS) products
- Rubisco (RBCS)
- Reactivity
- Arabidopsis thaliana, Synechocystis PCC 6803, Chlamydomonas, Potato, Soybean, Spinach
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Rubisco antibody is un-conjugated
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Application
- Western Blotting (WB)
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 53-55 / 53-55 kDa
- Purification
- serum
- Immunogen
- purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)
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anti-Rubisco (RBCS) antibody
RBCS Reactivity: Arabidopsis thaliana WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Rubisco (RBCS) (Large Subunit) antibodyRBCS Reactivity: Plant WB Host: Mouse Monoclonal 3G7 unconjugated
anti-Rubisco (RBCS) antibodyRBCS Reactivity: Plant WB Host: Mouse Polyclonal unconjugated
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- Application Notes
- Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard ECL(WB).
- Comment
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RbcS subunit is not detected by this antibody
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µL of sterile water.
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).
: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).
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: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).
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- Target
- Rubisco (RBCS)
- Alternative Name
- Rubisco (RBCS Products)
- Background
- Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
- Molecular Weight
- expected: 53-55 kDa, apparent: 53-55 kDa
- UniProt
- Q43832, P00875
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