Rpa12p (RPA12) antibody

Catalog No. ABIN190724

The Rabbit Polyclonal anti-Rpa12p antibody is suitable to detect Rpa12p in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for Rpa12p (RPA12) antibody (ABIN190724)

Target: Rpa12p (RPA12)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Purpose: Anti-A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.

Application Details

Application Notes: 1 : 2000 (WB)

Comment:

This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 143 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Rpa12p (RPA12)

Alternative Name: A12.2

Background:

Background: RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.

Additional information: This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.Protocol for isolation of cytosolic and nuclear fractions can be found here.

Molecular Weight: 13.6 kDa

Gene ID: 822191

UniProt: Q9LU97