Nitrogenase antibody

Catalog No. ABIN190742

There are 2+ publications for this product available. The Chicken Polyclonal anti-Nitrogenase antibody is suitable to detect Nitrogenase in samples from Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens and Vibrio sp.. It has been validated for IF, IHC and WB.

Quick Overview for Nitrogenase antibody (ABIN190742)

Target: Nitrogenase (Nifh)

Reactivity: Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens, Vibrio sp.

Host: Chicken

Clonality: Polyclonal

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Nitrogenase Antibody

Purpose: Anti-NifH | Nitrogenase iron protein

Cross-Reactivity (Details): Anabaena PCC7120, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii CS-505, Dolichospermum sp., Nostoc sp, Rhodopseudomonas palustris, Trichodesmium sp., nodules of Trifolium repens L., Vibrio natriegens ATCC 14048
Not reactive in: Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 as NifH protein is not present in those cyanobacterial species, Frankia sp.

Predicted Reactivity: Azotobacter vinelandii (Gram-),Bradyrhizobium japonicum,Cyanobacteria,Cyanothece ATCC51142,Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum,Enterobacter,genera,euryachaeotes,Klebsiella pneumonia,Magnetococcus sp.,Methanobacterium thermoautotrophicum,Methanococcus maripaludis,Methylobacterium sp.,Mesoorhizobium loti,Rhodopseudomonas palustris TIE-1 strain,alpha,gamma,beta proteobacteria,enterobacteria,low GC gram+,high GC gram +,able to fix atmoshperic nitrogen,Rhizobium meliloti

Purification: Immunogen affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78

Application Details

Application Notes: 1 : 500 (IHC), 6 μg/mL (IF), 1 : 2000 (WB)

Comment:

This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. The anti-NifH antibody will not show a strong reactivity to this target.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.36 µg/µL

Buffer: Immunogen affinity purified IgY in PBS pH 8 and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Nitrogenase antibody (ABIN190742)

Halimatul, Ehira, Awai: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).

Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

Target Details for Nitrogenase

Target: Nitrogenase (Nifh)

Background: Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.

Molecular Weight: 27 | 32.5 kDa

UniProt: Q2JP78, P26254, P33178, Q51296