PsbP antibody

Catalog No. ABIN190744

This Rabbit Polyclonal antibody specifically detects PsbP in WB. It exhibits reactivity toward Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Pinus banksiana, Spinach and Triticum aestivum. It has been mentioned in 2+ publications

Quick Overview for PsbP antibody (ABIN190744)

Target: PsbP

Reactivity: Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Pinus banksiana, Spinach, Triticum aestivum

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

Cross-Reactivity (Details): Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Not reactive in: Synechococcus sp. PCC 7942

Predicted Reactivity: Musa acuminata,Oryza sativa,Pinus monticola,Pisum sativum,Populus balsamifera,Solanum lycopersicum

Purification: Protein G purified

Immunogen: native

Application Details

Application Notes: 1 : 2000-1 : 5000 (WB)

Comment:

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Wang, Gu, Colby, Shindo, Balamurugan, Waldmann, Kaiser, van der Hoorn: "Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana." in: Nature chemical biology, Vol. 4, Issue 9, pp. 557-63, (2008) (PubMed).

Target Details

Target: PsbP

Background: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33- kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Molecular Weight: 28 | 23 kDa

UniProt: P12302