IL12B antibody (Internal Region)

Catalog No. ABIN190760

The Goat Polyclonal anti-IL12B antibody is suitable to detect IL12B in samples from Human. It has been validated for WB and ELISA.

Quick Overview for IL12B antibody (Internal Region) (ABIN190760)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IL12B Antibody

Binding Specificity: Internal Region

Purpose: IL12B / IL12p40

Sequence: QGKSKREKKD RVFTD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QGKSKREKKDRVFTD, from the internal region of the protein sequence according to NP_002178.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Human Liver, Skin and Tonsil lysates (calculated MW of 37.2 kDa according to NP_002178.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background: IL12B, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) , CLMF, CLMF2, IL-12B, NKSF, NKSF2, IL12, subunit p40, cytotoxic lymphocyte maturation factor 2, p40, interleukin 12, p40, interleukin 12B, in

Gene ID: 3593

NCBI Accession: NP_002178

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation