PTF1A antibody (Internal Region)

Catalog No. ABIN190768

This Goat Polyclonal antibody specifically detects PTF1A in WB and ELISA. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for PTF1A antibody (Internal Region) (ABIN190768)

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PTF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PTF1A Antibody

Binding Specificity: Internal Region

Purpose: PTF1A / PFT1-P48

Sequence: WTDEKQLKEQ N

Cross-Reactivity: Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-WTDEKQLKEQN, from the internal region of the protein sequence according to NP_835455.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa observe in Human Pancreas lysates and approx.38-40 kDa in lysates of cell lines Jurkat, U2OS and NIH3T3 ,(calculated MW of 35.0 kDa according to Human NP_835455.1 and 35.2 kDa according to Mouse NP_061279.2). Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PTF1A antibody (ABIN190768)

Holmstrom, Deering, Swift, Poelwijk, Mangelsdorf, Kliewer, MacDonald: "LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function." in: Genes & development, Vol. 25, Issue 16, pp. 1674-9, (2011) (PubMed).

Target Details for PTF1A

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Alternative Name: PTF1A

Background: PTF1A, PTF1-p48, pancreas specific transcription factor, 1a, bHLH transcription factor p48, class II bHLH protein PTF1A, exocrine pancreas-specific transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, pancreas transcription fact

Gene ID: 256297, 19213, 117034

NCBI Accession: NP_835455

Pathways: Retinoic Acid Receptor Signaling Pathway