Neurexin 1 antibody (C-Term)

Catalog No. ABIN190771

This Goat Polyclonal antibody specifically detects Neurexin 1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Neurexin 1 antibody (C-Term) (ABIN190771)

Target: Neurexin 1 (NRXN1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurexin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Neurexin 1 Antibody

Binding Specificity: C-Term

Purpose: Neurexin 1

Sequence: KEKQPSSAKS SNKN

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KEKQPSSAKSSNKN, from the C Terminus of the protein sequence according to NP_004792.1 , NP_620072.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60 kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDD

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Neurexin 1

Target: Neurexin 1 (NRXN1)

Alternative Name: NRXN1

Background: Neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.22998, KIAA0578, neurexin I

Gene ID: 9378, 18189, 60391

NCBI Accession: NP_004792, NP_620072

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development