ABHD5 antibody (Internal Region)

Catalog No. ABIN190850

The Goat Polyclonal anti-ABHD5 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect ABHD5 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for ABHD5 antibody (Internal Region) (ABIN190850)

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ABHD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ABHD5 Antibody

Binding Specificity: Internal Region

Purpose: CGI58 / ABHD5

Sequence: FPERPDLADQ DR

Specificity: This antibody is not expected to cross-react with ABHD4.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1 kDa according to NP_057090.2). Additional faint bands of 26 kDa and 18 kDa were also observed, and were successfully blocked by incubation with the immunizin

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ABHD5 antibody (ABIN190850)

Koltes, Spurlock: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." in: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).

Target Details for ABHD5

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Alternative Name: ABHD5

Background: ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2

Gene ID: 51099, 67469, 316122

NCBI Accession: NP_057090

Pathways: Lipid Metabolism