UBASH3A antibody (Internal Region)

Catalog No. ABIN190877

The Goat Polyclonal anti-UBASH3A antibody has been validated for ELISA and IHC. It is suitable to detect UBASH3A in samples from Human.

Quick Overview for UBASH3A antibody (Internal Region) (ABIN190877)

Target: UBASH3A (Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBASH3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UBASH3A Antibody

Binding Specificity: Internal Region

Purpose: STS2 / TULA (internal)

Sequence: QQCSTPDGKY YRPD

Specificity: This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QQCSTPDGKYYRPD, from the internal region of the protein sequence according to NP_061834.1, NP_001001895.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 48 kDa band in lysates of B-cell line Daudi after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBASH3A

Target: UBASH3A (Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A))

Alternative Name: UBASH3A

Background: TULA , STS2 CBL-interacting protein 4, UBASH3A, ubiquitin associated and SH3 domain containing, A , T-cell ubiquitin ligand protein, Suppressor of T-cell receptor signaling 2, CLIP4

Gene ID: 53347

NCBI Accession: NP_061834, NP_001001895