TCF3 antibody (Internal Region)

Catalog No. ABIN190880

The Goat Polyclonal anti-TCF3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TCF3 in samples from Human.

Quick Overview for TCF3 antibody (Internal Region) (ABIN190880)

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TCF3 Antibody

Binding Specificity: Internal Region

Purpose: TCF3 / ITF1

Sequence: KAPRARTSPD EDED

Specificity: This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KAPRARTSPDEDED, from the internal region of the protein sequence according to NP_003191.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Daudi (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3

Background: TCF3, ITF1, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), E2A, MGC129647, MGC129648 , E2A immunoglobulin enhancer-binding factor E12/E47, immunoglobulin transcription factor 1, kappa-E2-binding factor, transcription factor

Gene ID: 6929

NCBI Accession: NP_003191

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response