DGAT1 antibody (Internal Region)

Catalog No. ABIN190886

Product Details anti-DGAT1 Antibody

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DGAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: DGAT1

Sequence: QNSMKPFKDM DYS

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QNSMKPFKDMDYS, from the internal region of the protein sequence according to NP_036211.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 55 kDa band observed in Mouse Liver lysates (calculated MW of 56.8 kDa according to NP_034176.1). Recommended concentration: 0.01-0.1.0 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommende

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Alternative Name: DGAT1 (DGAT1 Products)

Synonyms: ARAT antibody, ARGP1 antibody, DGAT antibody, C75990 antibody, D15Ertd23e antibody, Dgat antibody, dgat1 antibody, wu:fd36g11 antibody, zgc:77691 antibody, DGAT1 antibody, ABX45 antibody, AS11 antibody, ATDGAT antibody, DIACYLGLYCEROL ACYLTRANSFERASE antibody, F27F23.24 antibody, RDS1 antibody, TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1 antibody, DDBDRAFT_0202877 antibody, DDBDRAFT_0304727 antibody, DDB_0202877 antibody, DDB_0304727 antibody, zgc:92327 antibody, diacylglycerol O-acyltransferase 1 antibody, diacylglycerol O-acyltransferase 1a antibody, diacylglycerol O-acyltransferase 1 L homeolog antibody, membrane bound O-acyl transferase (MBOAT) family protein antibody, diacylglycerol O-acyltransferase Dga1 antibody, diacylglycerol O-acyltransferase 1b antibody, DGAT1 antibody, Dgat1 antibody, dgat1a antibody, dgat1.L antibody, TAG1 antibody, dgat1 antibody, MGYG_02131 antibody, Tsp_02502 antibody, LOC100283803 antibody, dga1 antibody, dgat1b antibody

Background: DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase

Gene ID: 8694, 13350, 84497

NCBI Accession: NP_036211

Pathways: Hormone Transport