RAD9A antibody (RAD9 Homolog A (S. Pombe)) (C-Term)

Details for Product anti-RAD9A Antibody No. ABIN190894
Target
Binding Specificity
C-Term
26
25
25
24
19
15
15
15
11
8
8
7
7
7
7
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
226
78
68
6
3
2
2
1
1
Host
Goat
262
10
6
Clonality
Polyclonal
Conjugate
This RAD9A antibody is un-conjugated
21
21
19
12
12
12
10
9
9
9
9
9
8
8
8
8
8
Application
ELISA, Enzyme Immunoassay (EIA), Western Blotting (WB)
166
105
101
79
44
18
10
9
9
9
2
2
1
1
1
Options
Purpose RAD9A
Immunogen Peptide with sequence C-QGPSPVLAEDSEGE, from the C Terminus of the protein sequence according to NP_004575.1.
Blocking Peptide Blocking peptide for this product available: ABIN369636
Sequence QGPSPVLAED SEGE
Isotype IgG
Predicted Reactivity Human, Mouse, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name RAD9A (RAD9A Antibody Abstract)
Background RAD9A, RAD9 homolog A (S. pombe) , RAD9 , RAD9 (S. pombe) homolog, RAD9 homolog, RAD9 homolog (S. pombe), cell cycle checkpoint control protein
Gene ID 5883
NCBI Accession NP_004575
Pathways Positive Regulation of Response to DNA Damage Stimulus
Application Notes

DS WB Results: Approx 70 kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5 kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8,274(2):567-70., PMID: 9872989). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant RAD9A captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibody (ABIN190894) ABIN190894 (5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).
Image no. 2 for anti-RAD9 Homolog A (S. Pombe) (RAD9A) (C-Term) antibody (ABIN190894) ABIN190894 (0.3µg/ml) staining of A431cell lysate (35µg protein in RIPA buffer). Prim...