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DS WB Results: Approx 70 kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5 kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8,274(2):567-70., PMID: 9872989). Recommended concentration: 0.3-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant RAD9A captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml