GJB2 antibody (C-Term)

Catalog No. ABIN190896

This anti-GJB2 antibody is a Goat Polyclonal antibody detecting GJB2 in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for GJB2 antibody (C-Term) (ABIN190896)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GJB2 Antibody

Binding Specificity: C-Term

Purpose: GJB2 / Connexin 26

Sequence: YLLIRYCSGK SKKP

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YLLIRYCSGKSKKP, from the C Terminus of the protein sequence according to NP_003995.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25-26 kDa band observed in Human Colon, Prostate and Skin , Mouse Brain and in Mouse and Rat Heart lysates (calculated MW of 26.2 kDa according to Human NP_003995.2 and 26.4 kDa according to Mouse NP_032151.1 and Rat NP_001004099.1 )

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2

Background: GJB2, Connexin 26, gap junction protein, beta 2, 26 kDa (connexin 26) , CX26, DFNA3, DFNB1, HID, KID, NSRD1, PPK , gap junction protein beta 2, gap junction protein, beta 2, 26kD (connexin 26)

Gene ID: 2706, 14619, 394266

NCBI Accession: NP_003995

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization