CCKAR antibody (Internal Region)

Catalog No. ABIN190898

The Goat Polyclonal anti-CCKAR antibody has been validated for WB and ELISA. It is suitable to detect CCKAR in samples from Rat. There are 2+ publications available.

Quick Overview for CCKAR antibody (Internal Region) (ABIN190898)

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CCKAR Antibody

Binding Specificity: Internal Region

Purpose: CCKA Receptor (mouse)

Sequence: KFDASQKKSA KEKR

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KFDASQKKSAKEKR, from the internal region of the protein sequence according to NP_033957.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 75 kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009,2009:875167, PMID: 20011057.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CCKAR antibody (ABIN190898)

El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, (2010) (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, (2009) (PubMed).

Target Details for CCKAR

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Alternative Name: Cckar

Background: Cckar, cholecystokinin A receptor , AW106902

Gene ID: 12425, 24889

NCBI Accession: NP_033957

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour