LHCGR antibody (C-Term)

Catalog No. ABIN190901

The Goat Polyclonal anti-LHCGR antibody is suitable to detect LHCGR in samples from Human. It has been validated for IF, ELISA and FACS.

Quick Overview for LHCGR antibody (C-Term) (ABIN190901)

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LHCGR antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-LHCGR Antibody

Binding Specificity: C-Term

Purpose: LHCGR

Sequence: CQGTALLDKT RYTE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CQGTALLDKTRYTE, from the C Terminus of the protein sequence according to NP_000224.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 35 kDa band in K562 cell lysates after 0.1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please not

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LHCGR

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Alternative Name: LHCGR

Background: LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor

Gene ID: 3973

NCBI Accession: NP_000224

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, cAMP Metabolic Process, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling